Does the method work for my system?
More than likely Yes! We have designed kits to work in many systems, including: Amniotes, Angiosperms, Basal Arthropods, Insects, Mollusks, and Amphibians.
What information is needed to start a collaboration?
We just need to know your chosen Taxon group, its approximate genome size, your budget, and the number of samples that you are hoping to sequence.
Is RNase treatment necessary?
Yes! Excess RNA can result in high nanodrop concentration, making it seem like there is more DNA than is actually available. If RNA is not removed, it can also mislead quality measures (in a gel). The RNA looks like highly degraded DNA and can result in poor library construction due to correction for fragment size. Click here to see the results of different techniques for removing RNA from an extraction.
What DNA quality is required?
We ask for high molecular weight DNA. This can be visualized in a gel as a band near the sample well. These are often greater than 15kb in length. Click here for a sample gel.[/spoiler] [spoiler title=”I want to collaborate!”]
If you haven’t already, please view our project workflow before proceeding. If you foresee any issues with your samples, please note these problems in the description.[raw]
89 Chieftain Way, Biology Unit 1
Florida State University
Tallahassee, FL 32306-4295
Checklist before shipping DNA to FSU:
- Samples are treated with RNase during extraction
- Samples are run on a gel to check for size distribution
- Sample concentration checked with either Nanodrop spectrophotometer or Qubit fluorometer
- Gel is sent to FSU for confirmation of sample quality
- Gel approval is received. Samples can be shipped
- Sample template is filled out completely for all samples and shipped with samples and emailed to FSU